Human CXCL12 ELISA Kit (A1415)

Human CXCL12 ELISA Kit

Sandwich (quantitative)

Human CXCL12 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human CXCL12 in the samples. A monoclonal antibody specific for human CXCL12 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for CXCL12, and CXCL12 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CXCL12 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

62.50 - 4000 pg/ml

This kit can assay both natural and recombinant human CXCL12.

Human

This assay has shown no significant cross-reactivity.

102% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

C-X-C motif chemokine 12, hIRH, hSDF-1, Intercrine reduced in hepatomas, IRH, PBSF, Pre-B cell growth-stimulating factor, SDF-1, SDF1, SDF1A, SDF1B, Stromal cell-derived factor 1