Human CD40 ELISA Kit (A330000)

Human CD40 ELISA Kit

Human CD40 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human CD40 in cell culture supernates, serum, edta plasma, heparin plasma.

Sandwich (quantitative)

Human CD40 ELISA Kit (A330000) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human CD40 in cell culture supernates, serum, edta plasma, heparin plasma. An antibody specific for CD40 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CD40 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-CD40 Antibody, which binds the captured CD40 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CD40 captured in each well. The concentration of CD40 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Cell culture supernates, serum, EDTA plasma, heparin plasma.

1.84 pg/ml

15.63-1000 pg/ml

4 hours

100µl

Human

Intra-assay CV: <4.1%, Inter-assay CV: <7.1%.

Information online is representative. Always refer to the Product Manual inside the kit. This ELISA kits uses Anti-CD40 Antibody (A329217) as the capture antibody, Anti-CD40 Antibody (A329218) as the detection antibody, and Recombinant Human CD40 Protein (C-terminal His Tag) (A330337) as the protein standard.

Shipped at 4°C. Unopened kits can be stored at +4°C for 12 months. Once opened products must be used within 1 month.

This product is for research use only. It is not intended for diagnostic or therapeutic use.