Human CCL5 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human RANTES in the samples. A monoclonal antibody specific for human RANTES has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for RANTES, and RANTES present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of RANTES bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
0.53 pg/ml
Product Range
15.63 - 1000 pg/ml
Specificity
This kit can assay both natural and recombinant human RANTES.
Reactivity
Human
Cross Reactivity
This assay has shown no significant cross-reactivity.
Recovery
97% (Average Recovery from Serum Samples).
Storage
Store at 2 - 8°C.
Synonyms
C-C motif chemokine 5, D17S136E, EoCP, Eosinophil chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, TCP228
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
