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Human CCL17 ELISA Kit (A531)

$795

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.
Name
Human CCL17 ELISA Kit
Assay Type
Sandwich (quantitative)
Principle of Assay
This CCL17 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-CCL17 Antibody. Standards or samples are then added to the microtire plate wells and CCL17, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of CCL17 present in the sample, Anti-CCL17 Antibody (Biotin) is added to each well to "sandwich" the CCL17 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CCL17 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.
Sensitivity
< 1 pg/ml
Product Range
31.2-2000 pg/ml
Reactivity
Human
Storage
Store at 2-8°C for 4 months or at -20°C for 8 months.
Synonyms
C-C motif chemokine 17, CC chemokine TARC, SCYA17, Small-inducible cytokine A17, TARC, Thymus and activation-regulated chemokine
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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