Human Cathepsin B ELISA Kit (A119843)

Human Cathepsin B ELISA Kit

Human Cathepsin B ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Cathepsin B in serum, plasma, cell culture supernates, cell lysates, tissue homogenates and other biological fluids.

Sandwich (quantitative)

Human Cathepsin B ELISA Kit (A119843) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Cathepsin B in serum, plasma, cell culture supernates, cell lysates, tissue homogenates and other biological fluids. An antibody specific for Cathepsin B has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Cathepsin B present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Cathepsin B Antibody, which binds the captured Cathepsin B present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Cathepsin B captured in each well. The concentration of Cathepsin B can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

0.02 ng/ml

0.31-20 ng/ml

4 hours

100µl

Human

Intra-assay CV: <3.5%, Inter-assay CV: <6.8%.

Information online is representative. Always refer to the Product Manual inside the kit. This ELISA kits uses Anti-Cathepsin B Antibody (A329174) as the capture antibody, Anti-Cathepsin B Antibody (A329175) as the detection antibody, and Recombinant Human Cathepsin B Protein (C-terminal His Tag) (A330191) as the protein standard.

Shipped at 4°C. Unopened kits can be stored at +4°C for 12 months. Once opened products must be used within 1 month.

APP secretase, APPS, Cathepsin B1, CPSB, CTSB