Principle of Assay
Human Apolipoprotein A1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Apo A1 in the samples. A monoclonal antibody specific for human Apo A1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Apo A1, and Apo A1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Apo A1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
