Human Adiponectin ELISA Kit (A1382)

Human Adiponectin ELISA Kit

Sandwich (quantitative)

Human Adiponectin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Adiponectin in the samples. A monoclonal antibody specific for human Adiponectin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for Adiponectin, and Adiponectin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Adiponectin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

Cell culture supernatant, serum and plasma.

62.50 - 4000 pg/ml

This kit can assay both natural and recombinant human Adiponectin.

Human

This assay has shown no significant cross-reactivity.

99% (Average Recovery from Serum Samples).

Store at 2 - 8°C.

30 kDa adipocyte complement-related protein, ACDC, ACRP30, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein, ADIPOQ, Adipose most abundant gene transcript 1 protein, apM-1, APM1, GBP28, Gelatin-binding protein