Histone H2B Cell Based ELISA Kit (A103372)

Histone H2B Cell Based ELISA Kit

Cell-based (qualitative)

This Histone H2B Cell Based ELISA Kit allows for the detection of Histone H2B and the effects that certain stimulation conditions have on Histone H2B expression in different cell lines. Qualitative determination of Histone H2B concentration is achieved by an indirect ELISA format. In essence, the Histone H2B is captured by Anti-Histone H2B Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Histone H2B Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Colorimetric

Adherent cells and suspension cells.

4 hours 30 minutes

Human, Mouse

Store at + 4°C. Stable for 6 months.

H2B K, H2B type 12, H2B-clustered histone 12 like, H2B-clustered histone 17, H2B-clustered histone 18, H2B-clustered histone 21, H2B-clustered histone 26, H2B-clustered histone 3, H2B-clustered histone 5, H2B-clustered histone 9, H2B.S histone 1, H2B.U histone 1, H2B/a, H2B/b, H2B/c, H2B/d, H2B/e, H2B/f, H2B/g, H2B/h, H2B/j, H2B/k, H2B/l, H2B/n, H2B/q, H2B/s, H2BC12, H2BC12L, H2BC13, H2BC14, H2BC15, H2BC17, H2BC18, H2BC21, H2BC26, H2BC3, H2BC4, H2BC5, H2BC9, H2BFB, H2BFC, H2BFD, H2BFE, H2BFF, H2BFH, H2BFJ, H2BFL, H2BFN, H2BFQ, H2BFS, H2BFT, H2BS1, H2BU1, HIRA-interacting protein 1, HIRA-interacting protein 2, HIRIP1, HIRIP2, HIST1H2BB, HIST1H2BC, HIST1H2BD, HIST1H2BH, HIST1H2BK, HIST1H2BL, HIST1H2BM, HIST1H2BN, HIST1H2BO, HIST2H2BE, HIST2H2BF, HIST3H2BB, Histone H2B type 1-B, Histone H2B type 1-C/E/F/G/I, Histone H2B type 1-D, Histone H2B type 1-H, Histone H2B type 1-K, Histone H2B type 1-L, Histone H2B type 1-M, Histone H2B type 1-N, Histone H2B type 1-O, Histone H2B type 2-E, Histone H2B type 2-F, Histone H2B type 3-B, Histone H2B type F-S, Histone H2B-GL105, Histone H2B.1, Histone H2B.1 A, Histone H2B.1 B, Histone H2B.2, Histone H2B.a, Histone H2B.b, Histone H2B.c, Histone H2B.d, Histone H2B.e, Histone H2B.f, Histone H2B.g, Histone H2B.h, Histone H2B.j, Histone H2B.k, Histone H2B.l, Histone H2B.n, Histone H2B.q, Histone H2B.s