Chicken Testosterone ELISA Kit (A327135)

$582

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.
Name
Chicken Testosterone ELISA Kit
Description
Chicken Testosterone ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of chicken Testosterone in serum and plasma.
Assay Type
Competitive (quantitative)
Principle of Assay
Chicken Testosterone ELISA Kit (A327135) employs the competitive enzyme immunoassay technique for the quantitative measurement of chicken Testosterone in serum and plasma. The 96-well microtiter plate has been pre-coated with Testosterone antigen. During the incubation, Testosterone present in the samples or standards competes with the fixed amount of immobilized Testosterone for binding sites on the Biotinylated Anti-Testosterone Antibody. The more Testosterone present in a sample or standard, the less Biotinylated Anti-Testosterone Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Testosterone Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Testosterone present in each sample or standard. The concentration of Testosterone can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colormetric
Instrument
Colorimetric Microplate Reader
Sample Type
Serum and plasma.
Sensitivity
0.13 ng/ml
Product Range
0.31-20 ng/ml
Assay Time
1h 30m
Reactivity
Chicken
Cross Reactivity
No significant cross-reactivity or interference between chicken Testosterone and analogues was observed.
Recovery
Precision
Intra-assay CV: 5.15%, Inter-assay CV: 4.76%
General Notes
Information online is representative. Always refer to the Product Manual inside the kit.
Storage
Shipped at +4°C. Store short term at +4°C and long term at +4°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Chicken Testosterone ELISA Kit (A327135)? Please let us know so that we can list the citation on this page.