beta Catenin (phospho Tyr489) Cell Based ELISA Kit (A103765)

Name: beta Catenin (phospho Tyr489) Cell Based ELISA Kit
Brand: Antibodies.com
SKU: A103765
Price: 670.0 USD
Availability: InStock

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$670

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-8 business days.

Specifications

Name

beta Catenin (phospho Tyr489) Cell Based ELISA Kit

Assay Type

Cell-based (qualitative)

Principle of Assay

This beta Catenin (phospho Tyr489) Cell Based ELISA Kit allows for the detection of beta Catenin (phospho Tyr489) and the effects that certain stimulation conditions have on beta Catenin (phospho Tyr489) expression in different cell lines. Qualitative determination of beta Catenin (phospho Tyr489) concentration is achieved by an indirect ELISA format. In essence, beta Catenin (phospho Tyr489) is captured by Anti-beta Catenin (phospho Tyr489) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-beta Catenin Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.

Platform

Microplate

Detection Type

Fluorometric

Product Range

> 5000 Cells

Reactivity

Human, Mouse, Rat

Storage

Store at + 4°C. Stable for 6 months.

Synonyms

Beta-catenin, Catenin beta-1, CTNNB, CTNNB1

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - beta Catenin (phospho Tyr489) Cell Based ELISA Kit (A103765)

Western blot analysis of extracts from COS7 cells treated with UV 15', using Anti-Catenin-beta (phospho Tyr489) Antibody.

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beta Catenin (phospho Tyr489) Cell Based ELISA Kit (A103765)

A diagram showing the protocol for beta Catenin (phospho Tyr489) Cell Based ELISA Kit.

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beta Catenin (phospho Tyr489) Cell Based ELISA Kit (A103765)

A summary of the protocol for beta Catenin (phospho Tyr489) Cell Based ELISA Kit.

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Western Blot - beta Catenin (phospho Tyr489) Cell Based ELISA Kit (A103765)

The mouse monoclonal antibody to GAPDH is used as a positive control in beta Catenin (phospho Tyr489) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.

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