FAM (fluorescein) is a bright fluorophore which is compatible with various fluorescence detection instruments. FAM is a universal dye that is useful for microscopy, qPCR and many other methods as well as for FRET-based and fluorescence polarization based binding assays. FAM maleimide is a thiol reactive dye for the labeling of proteins, peptides, and other thiolated molecules.
Absorption Maxima
494 nm
Extinction Coefficient
75000 M-1cm-1
Emission Maxima
520 nm
Fluorescence Quantum Yield
0.9
CF260
0.20
CF280
0.17
Mass Spec M+ Shift after Conjugation
498.4
Purity
95% (by 1H NMR and HPLC-MS).
Molecular Formula
C27H18N2O8
Molecular Weight
498.44 kDa
Product Form
Yellow solid.
Solubility
Good in DMSO and DMF.
Storage
Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
