dsGreen for Real-Time PCR (A270197)

dsGreen for Real-Time PCR

dsGreen, an analog of SYBR® Green I, is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allows the use of dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen - unlabeled primers are sufficient. Unlike other preparations of dsGreen for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:. Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot. PCR tested preparation - quality guaranteed. Low fluorescence background - high fluorescence intensity gain.

454 nm

73000 M^-1cm^-1

0,8

95% (by ¹H NMR, HPLC-MS, and PCR testing).

100X

Light orange solution.

Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light.

This product is for research use only. It is not intended for diagnostic or therapeutic use.