Cyanine 3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine 3 NHS ester which does not require use of any co-solvent. Cyanine 3 NHS ester is a replacement for NHS esters of Cy3® and DyLight 549.
Absorption Maxima
555 nm
Extinction Coefficient
150000 M-1cm-1
Emission Maxima
570 nm
Fluorescence Quantum Yield
0.31
CAS Number
2632339-91-2
CF260
0.04
CF280
0.09
Mass Spec M+ Shift after Conjugation
474.2
Purity
> 95% (by 1H NMR and HPLC-MS).
Molecular Formula
C34H40N3BF4O4
Molecular Weight
641.5 kDa
Product Form
Red powder.
Solubility
Poorly soluble in water (2.3 mM = 1.5 g/L). Soluble in organic solvents (DMF, DMSO, dichloromethane).
Storage
Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
