Succinate Dehydrogenase Activity Assay Kit (A319738)

Overview
Specifications

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Succinate Dehydrogenase Activity Assay Kit

Description

This Succinate Dehydrogenase Activity Assay Kit is a quantitative assay kit for measuring Succinate Dehydrogenase activity in animal and plant tissues, cells and bacteria.

Applications

Functional Studies

Assay Type

Quantitative

Principle of Assay

This kit provides a simple method for detecting succinate dehydrogenase (SDH) activity in animal and plant tissues, cells, and bacteria. The detection principle is based on SDH catalyzed dehydrogenation of succinate to produce fumarate. Phenazine dimethyl ester sulphuric acid (PMS) couples the dehydrogenation to the reduction of 2,6-Dichlorophenolindophenol (2,6-DCPIP). 2,6-DCPIP has an absorption peak at 605 nm. The reduction of 2,6-DCPIP is followed by measuring the change in absorbance at 605 nm, from which the SDH activity in samples can be calculated.

Platform

96-Well Microplate, Microcuvette

Sample Type

Animal and plant tissues, cells, and bacteria.

Components

Extraction Buffer, Reagent I, Reagent II, Reagent III, Reagent IV

Storage

Shipped at 4°C. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!

General Notes

96-well microplate / microglass cuvette is required.

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

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Succinate Dehydrogenase Activity Assay Kit (A319738)

Shipping Information

Specifications