Confocal and long-term super-resolution imaging (such as SIM, TIRF, STORM and PALM).
Principle of Assay
Lyso Red is a fluorescent probe with Red fluorescence that can specifically label lysosomes in living mammalian cells. It has strong water solubility and pH stability; its maximum excitation wavelength is 651 nm, and its maximum emission wavelength is 672 nm. Especially suitable for Confocal and long-term super-resolution imaging (such as SIM, TIRF, STORM and PALM). Low levels of cytotoxicity.
Long time series images (a, c) and corresponding fluorescence bleaching curves (b, d) were obtained by using Live-cell Lyso-traker Kit (Red Fluorescence) (A319618) and a commercially available Lyso Tracker. HiS-SIM and spinning disc confocal microscopy analysis using U-2 OS cells. Scale bars: 5 µM.
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