Principle of Assay
This kit provides a convenient tool for detection of malondialdehyde (MDA) present in a variety of samples. Malondialdehyde (MDA) can be condensed with thiobarbituric acid under acidic and high temperature conditions to produce the brown-red trimethyltran (3,5, 5-trimethyloxazol-2, 4-dione) with a maximum absorption wavelength of 532 nm. Meanwhile, the absorbance at 600 nm is measured to eliminate the interference of sucrose. MDA content is calculated from the difference between the absorbance at 532 nm and 600 nm.
