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Cell Counting Kit (A319631)

$175

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.
Name
Cell Counting Kit
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
This kit allows very convenient assays by utilizing the highly water-soluble tetrazolium salt-WST-8. [2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. This kit is designed to detect cell proliferation and cell toxicity based on WST-8 being reduced by dehydrogenases in cells to give an orange colored product (formazan). The amount of the formazan in cells is directly proportional to the number of living cells. The product (formazan) produced by WST-8 is water soluble, no organic solvents or isotopes required. And the formazan is stable and safe. The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.
Platform
96-Well Microplate
Sample Type
Cells
Components
CCK-8 Solution
Storage
Shipped at 4°C. Store at -20°C in the dark for long-term storage. Repeated freeze/thaw cycles cause an increase in the background, which interferes with the assay. Please store the kit at 4°C for frequent use. Do not use past expiration date!
General Notes
Since this assay is based on the detection of dehydrogenase activity in viable cells, conditions or chemicals that affect dehydrogenase activity in viable cells may cause a discrepancy between the actual viable cell number and the cell number determined using this assay.A 96-well clear flat bottom microplate is required.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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