This Caspase-3 Assay Kit (Colorimetric) is a quantitative assay kit for measuring Caspase-3 activity in animal tissues and cells.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Caspase 3 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-DEVD-pNA (acetyl-Asp-Glu-Val-Asp p-nitroanilide) by caspase 3, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm.