Anti-Weri-RB1 Retinoblastoma Cells Aptamer (Biotin) (A321243)

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 30-45 business days.
Name
Anti-Weri-RB1 Retinoblastoma Cells Aptamer (Biotin)
Description
Synthetic DNA aptamer to Weri-RB1 Retinoblastoma Cells (Biotin).
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Binding Buffer: 5% Fetal Bovine Serum with 1 mg/ml Bovine Serum Albumin and 0.1 mg/mL Yeast tRNA. Binding Temperature: 20°C.
Sequence
5'-dTpdApdGpdGpdGpdApdApdGpdApdGpdApdApdGpdGpdApdCpdApdTpdApdTpdGpdApdTpdTpdCpdApdApdApdGpdTpdApdApdCpdTpdCpdTpdGpdTpdCpdApdCpdApdGpdTpdApdCpdApdApdTpdApdGpdApdTpdTpdCpdTpdCpdApdTpdTpdApdTpdCpdApdTpdTpdGpdApdCpdTpdApdGpdTpdApdCpdApdTpdGpdApdCpdCpdApdCpdTpdTpdGpdAp-3'
Aptamer Length
87 nt
Aptamer Molecular Weight (Da)
26,946.28 Da
Aptamer Affinity
Affinity (Kd) = 49.41?±?7.87 nM
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Weri-RB1 Retinoblastoma Cells Aptamer (Biotin) (A321243) is a replica of the aptamer described in PMID: 36171412.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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