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Anti-MM.1S Multiple Myeloma Cells Aptamer [TY04] (Biotin) (A321011)

1 Citation
Secondary Structure - Anti-MM.1S Multiple Myeloma Cells Aptamer [TY04] (Biotin) (A321011) - Antibodies.com
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 30-45 business days.

  • Specifications

Name
Anti-MM.1S Multiple Myeloma Cells Aptamer [TY04] (Biotin)
Description
Synthetic DNA aptamer to MM.1S Multiple Myeloma Cells [TY04] (Biotin).
Specificity
Aptermer TY04 inhibits the growth of MM cell lines, including MM.1S, NCI-H929, KM3, and OPM2.
Aptamer ID
TY04
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
Yeast tRNA 0.1mg/ml and 1 mg/ml Bovine Serum Albumin. Binding Temperature: 37°C.
Sequence
5'-dApdTpdCpdGpdTpdCpdTpdGpdCpdTpdCpdCpdGpdTpdCpdCpdApdApdTpdApdTpdApdTpdCpdApdApdApdGpdGpdCpdGpdApdApdTpdTpdTpdTpdGpdTpdCpdApdApdGpdGpdTpdGpdTpdTpdApdApdApdCpdGpdApdTpdApdGpdTpdCpdCpdCpdTpdApdCpdCpdTpdTpdTpdGpdGpdTpdGpdTpdGpdApdGpdGpdTpdCpdGpdTpdGpdCp-3'
Aptamer Length
83 nt
Aptamer Molecular Weight (Da)
25,587.67 Da
Aptamer Affinity
IC50 = 3.89 µM
Extinction Coefficient
794400 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-MM.1S Multiple Myeloma Cells Aptamer [TY04] (Biotin) (A321011) is a replica of the aptamer described in PMID: 24792887.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Secondary Structure - Anti-MM.1S Multiple Myeloma Cells Aptamer [TY04] (Biotin) (A321011) - Antibodies.comEnlarge Image

Anti-MM.1S Multiple Myeloma Cells Aptamer [TY04] (Biotin) (A321011)

Secondary structure of Anti-MM.1S Multiple Myeloma Cells Aptamer [TY04] (Biotin) (A321011), with a dG of -3.059 kcal/mol.

  • Product Citations (1)

PubMed LogoView Publication
Aptamer TY04 inhibits the growth of multiple myeloma cells via cell cycle arrest
This publication cites Anti-MM.1S Multiple Myeloma Cells Aptamer [TY04] (Biotin) (A321011).
Article Abstract

The aptamer TY04 is a single-stranded DNA. However, its biological function has not been elucidated. Here, we found that TY04 specifically bound to multiple myeloma cells MM.1S, and some membrane proteins on the surface of MM.1S cells constituted the target molecules of TY04. TY04 inhibited the growth of multiple myeloma cell lines, induced cell cycle arrest in mitosis, and resulted in a significant accumulation of binucleated cells. Following TY04 treatment, a concomitant increase in CDK1 and cyclin B1 expression occurred. In addition, TY04 treatment also resulted in a significant downregulation of ?-tubulin. Considering the unique advantages of aptamers, TY04 shows great potential as a drug candidate to treat multiple myeloma.

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