Anti-L-Arginine Aptamer [12-28 hairpin] (Biotin) (A320945)

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 30-45 business days.
Name
Anti-L-Arginine Aptamer [12-28 hairpin] (Biotin)
Description
Synthetic DNA aptamer to L-Arginine [12-28 hairpin] (Biotin).
Specificity
The aptamer's affinity for argininamide and agmatine is 20-fold more than for arginine. Affinities for D-arginine, guanidine, and ethylguanidine are about the same.
Aptamer ID
12-28 hairpin
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatamer Refolding Conditions
Denatured at 95°C and then cooled to 4°C for over 2 hours.
Apatmer Binding Conditions
Buffer for CD binding assay: 10mM Sodium Phosphate Buffer, pH 7.5, with 100mM Sodium Chloride. Binding Temperature: 4°C.
Sequence
5'-dGpdGpdGpdApdTpdCpdGpdApdApdApdCpdGpdTpdApdGpdCpdGpdCpdCpdTpdTpdCpdGpdApdTpdCpdCpdCp-3'
Aptamer Length
28 nt
Aptamer Molecular Weight (Da)
8,574.6 Da
Aptamer Affinity
Affinity (Kd) = ~2.5 nM
Extinction Coefficient
264800 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-L-Arginine Aptamer [12-28 hairpin] (Biotin) (A320945) is a replica of the aptamer described in PMID: 8846773.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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