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Anti-Insulin Aptamer [IBA] (Biotin) (A321027)

1 Citation
Secondary Structure - Anti-Insulin Aptamer [IBA] (Biotin) (A321027) - Antibodies.com
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Product Datasheet
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 30-45 business days.

  • Specifications

Name
Anti-Insulin Aptamer [IBA] (Biotin)
Description
Synthetic DNA aptamer to Insulin [IBA] (Biotin).
Specificity
Specificity tested against Streptavidin, Avidin, and BSA.
Aptamer ID
IBA
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatmer Binding Conditions
50mM Tris-HCl Buffer, 10mM Potassium Chloride, and 100mM Sodium Chloride. Binding Temperature: 23°C.
Sequence
5'-dGpdGpdTpdGpdGpdTpdGpdGpdGpdGpdGpdGpdGpdGpdTpdTpdGpdGpdTpdApdGpdGpdGpdTpdGpdTpdCpdTpdTpdCp-3'
Aptamer Length
30 nt
Aptamer Molecular Weight (Da)
9,493.19 Da
Extinction Coefficient
288200 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Insulin Aptamer [IBA] (Biotin) (A321027) is a replica of the aptamer described in PMID: 21874167.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Secondary Structure - Anti-Insulin Aptamer [IBA] (Biotin) (A321027) - Antibodies.comEnlarge Image

Anti-Insulin Aptamer [IBA] (Biotin) (A321027)

Secondary structure of Anti-Insulin Aptamer [IBA] (Biotin) (A321027), with a dG of 0.158 kcal/mol.

  • Product Citations (1)

Insulin-binding aptamer-conjugated graphene oxide for insulin detection
This publication cites Anti-Insulin Aptamer [IBA] (Biotin) (A321027).
Article Abstract

This paper describes a simple and sensitive aptamer/graphene oxide (GO) based assay for insulin detection. GO can protect DNA from nuclease cleavage, but aptamers can be detached from the GO surface by specific target binding. This exposes the aptamers to enzymatic cleavage and releases the target for a new cycle. Cycling of targets leads to significant signal amplification and low LOD.

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