Anti-Ethanolamine Aptamer [14.3] (Biotin) (A320808)

Name: Anti-Ethanolamine Aptamer [14.3] (Biotin)
Brand: Antibodies.com
SKU: A320808
Price: 755.0 USD
Availability: InStock

1 Citation

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Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 30-45 business days.

Specifications

Name

Anti-Ethanolamine Aptamer [14.3] (Biotin)

Description

Synthetic DNA aptamer to Ethanolamine [14.3] (Biotin).

Aptamer ID

14.3

Conjugate

Biotin

Aptamer Resuspension

Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.

Apatmer Binding Conditions

Binding Buffer: 20mM Tris Buffer, pH 8, with 100mM Sodium Chloride, 2mM Magnesium Chloride, 5mM Potassium Chloride, 1mM Calcium Chloride, and 0.2% Tween 20. Binding Temperature: 21°C.

Sequence

5'-dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdTpdGpdApdGpdGpdCpdGpdGpdGpdTpdGpdGpdGpdTpdGpdGpdGpdTpdTpdGpdApdApdTpdApdTpdGpdCpdTpdGpdApdTpdTpdApdCpdCpdCpdCpdApdTpdCpdGpdGpdApdGpdApdApdCpdGpdTpdTpdApdApdGpdGpdCpdGpdCpdTpdTpdCpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp-3'

Aptamer Length

96 nt

Aptamer Molecular Weight (Da)

29,786.42 Da

Aptamer Affinity

Affinity (Kd) = 6 nM

Extinction Coefficient

940500 M^-1cm^-1

Purification

HPLC

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from nuclease-free water (no residual salts).

Storage

Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.

Product Notes

Anti-Ethanolamine Aptamer [14.3] (Biotin) (A320808) is a replica of the aptamer described in PMID: 16289104.

General Notes

Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days. See reference for more information etc. This aptamer is manufactured and released using quantitative quality control methods that evaluate purity and DNA sequence. Therefore, we recommend optimizing the experimental conditions for the aptamer in the chosen assay (western blotting, immunofluorescence, immunohistochemistry and co-IP) .

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Anti-Ethanolamine Aptamer [14.3] (Biotin) (A320808)

Secondary structure of Anti-Ethanolamine Aptamer [14.3] (Biotin) (A320808), with a dG of -16.18 kcal/mol.

Product Citations (1)

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In vitro selection of DNA aptamers binding ethanolamine

This publication cites Anti-Ethanolamine Aptamer [14.3] (Biotin) (A320808).

Article Abstract

We have identified aptamers (synthetic oligonucleotides) binding to the very small molecule ethanolamine with high affinity down to the low nanomolar range. These aptamers were selected for their ability to bind to ethanolamine immobilised on magnetic beads, from an 96mer library of initially about 1 x 10(16) randomised ssDNA molecules. The dissociation constants of these aptamers range between K(D)=6 and K(D)=19 nmol L(-1). The aim of the development of ethanolamine aptamers is their use for the detection of this substance in clinical and environmental analysis. Ethanolamine is associated with several diseases. Moreover, ethanolamine and its derivatives di- and tri-ethanolamine are used in chemical and cosmetic industries. The use of biosensors with ethanolamine aptamer as new molecular recognition element could be an innovative method for an easy and fast detection of ethanolamine.

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Anti-Ethanolamine Aptamer [14.3] (Biotin) (A320808)

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Specifications

Scientific Validation DataValidation Data (1)

Anti-Ethanolamine Aptamer [14.3] (Biotin) (A320808)

Product Citations (1)