Anti-Catalase (Bovine) Aptamer (Biotin) (A320880)

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 30-45 business days.
Name
Anti-Catalase (Bovine) Aptamer (Biotin)
Description
Synthetic DNA aptamer to Catalase (Bovine) (Biotin).
Specificity
At least a 100-fold decrease in affinity to lysozyme, trypsinogen, chymotrypsinogenA, and myoglobin relative to the target.
Reactivity
Bovine
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatamer Refolding Conditions
Denatured at 94°C for 10 min and then cooled at 0.5°C/s to 20°C.
Apatmer Binding Conditions
CE Selection Buffer (TGK): 25mM Tris and 5mM Potassium Phosphate Buffer, pH 8.3, with 192mM Glycine. CE Run Buffer: 3X TGK.
Sequence
5'-dCpdTpdTpdCpdTpdGpdCpdCpdCpdGpdCpdCpdTpdCpdCpdTpdTpdCpdCpdGpdApdCpdCpdTpdApdGpdCpdApdGpdTpdGpdGpdApdCpdApdTpdGpdTpdGpdGpdCpdApdGpdGpdGpdTpdGpdApdApdGpdTpdGpdGpdCpdApdTpdCpdGpdTpdCpdGpdGpdApdGpdApdCpdGpdApdGpdApdTpdApdGpdGpdCpdGpdGpdApdCpdApdCpdTp-3'
Aptamer Length
82 nt
Aptamer Molecular Weight (Da)
25,356.41 Da
Aptamer Affinity
Affinity (Kd) = 237 nM
Extinction Coefficient
774800 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Catalase (Bovine) Aptamer (Biotin) (A320880) is a replica of the aptamer described in PMID: 22965726.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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