Anti-Adenosine Aptamer (Biotin) (A321074)

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 30-45 business days.
Name
Anti-Adenosine Aptamer (Biotin)
Description
Synthetic DNA aptamer to Adenosine (Biotin).
Specificity
This aptamer has high specificity to adenosine and low specificity to ATP, AMP, Guanosine, Cytidine, Uridine and Theophylline.
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatamer Refolding Conditions
Denatured at 75°C for 5 min and then cooled to room temperature for 20-30 min.
Apatmer Binding Conditions
Column Buffer: 20mM Tris-HCl Buffer, pH 7.6, with 300mM Sodium Chloride and 5mM Magnesium Chloride. Binding Temperature: 37°C.
Sequence
5'-dGpdTpdApdTpdTpdGpdCpdGpdGpdApdGpdGpdApdApdGpdGpdTpdTpdTpdTpdTpdApdApdCpdCpdTpdTpdCpdGpdGpdGpdGp-3'
Aptamer Length
32 nt
Aptamer Molecular Weight (Da)
9,966.5 Da
Aptamer Affinity
Affinity (Kd) = 12.7-16.9 nM
Extinction Coefficient
311000 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Adenosine Aptamer (Biotin) (A321074) is a replica of the aptamer described in PMID: 34084332.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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