Anti-Caspase 9 (phospho Thr125) Antibody (A93473)

Name: Anti-Caspase 9 (phospho Thr125) Antibody
Brand: Antibodies.com
SKU: A93473
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to Caspase 9 (phospho Thr125) for WB, IHC and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Caspase 9 (phospho Thr125) Antibody

Description

Rabbit polyclonal antibody to Caspase 9 (phospho Thr125).

Specificity

This antibody detects endogenous levels of Caspase 9 only when phosphorylated at Thr125.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, ELISA: 1:10000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human Caspase 9 around the phosphorylation site of Thr125 (amino acids 91-140).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

46kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, CASP9_HUMAN, Caspase-9 subunit p10, ICE-LAP6, ICE-like apoptotic protease 6, ICELAP6, MCH6

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Caspase 9 (phospho Thr125) Antibody (A93473)

Western blot analysis of lysates from HeLa cells treated with TNF 20ng/ml 5'+calyculin A 50ng/ml 5' using Anti-Caspase 9 (phospho Thr125) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-Caspase 9 (phospho Thr125) Antibody (A93473)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Anti-Caspase 9 (phospho Thr125) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.