Anti-Caspase-7 Antibody (A13487)

Name: Anti-Caspase-7 Antibody
Brand: Antibodies.com
SKU: A13487
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Caspase-7 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Caspase-7 Antibody

Description

Rabbit polyclonal antibody to Caspase-7.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human Caspase-7 (NP_001218.1).

Sequence

IYGKDGVTPIKDLTAHFRGDRCKTLLEKPKLFFIQACRGTELDDGIQADSGPINDTDANPRYKIPVEADFLFAYSTVPGYYSWRSPGRGSWFVQALCSILE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

35 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apoptotic protease Mch-3, Apoptotic protease MCH3, CASP-7, CASP7, CASP7_HUMAN, Caspase 7, Caspase 7 apoptosis related cysteine peptidase, Caspase-7 subunit p11, Caspase7, CMH 1, CMH-1, CMH1, ICE LAP3, ICE-LAP3, ICE-like apoptotic protease 3, LICE2, MCH3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (10)

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Figure 1: Western Blot - Anti-Caspase-7 Antibody (A13487)

Western blot analysis of extracts of Rat liver, using Anti-Caspase-7 Antibody (A13487) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-Caspase-7 Antibody (A13487)

Western blot analysis of extracts of Mouse liver, using Anti-Caspase-7 Antibody (A13487) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 3: Immunohistochemistry - Anti-Caspase-7 Antibody (A13487)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Caspase-7 Antibody (A13487) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Caspase-7 Antibody (A13487)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Caspase-7 Antibody (A13487) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.