Anti-Caspase 6 (phospho Ser257) Antibody (A94575)

Name: Anti-Caspase 6 (phospho Ser257) Antibody
Brand: Antibodies.com
SKU: A94575
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to Caspase 6 (phospho Ser257) for WB, IHC, IF and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Caspase 6 (phospho Ser257) Antibody

Description

Rabbit polyclonal antibody to Caspase 6 (phospho Ser257).

Specificity

This antibody detects endogenous levels of Caspase 6 only when phosphorylated at Ser257.

Applications

WB, IHC, IF, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:40000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human Caspase 6 around the phosphorylation site of Ser257 (amino acids 223-272).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.

Molecular Weight

33kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apoptotic protease Mch-2, Apoptotic protease MCH2, CASP-6, CASP6, CASP6_HUMAN, Caspase 6, Caspase 6 apoptosis related cysteine protease, Caspase 6, apoptosis related cysteine peptidase, Caspase-6 subunit p11, Caspase-6 subunit p20, Mch2, OTTHUMP00000162742, OTTHUMP00000162743

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Caspase 6 (phospho Ser257) Antibody (A94575)

Western blot analysis of lysates from 293 cells treated with Etoposide 25uM 60' using Anti-Caspase 6 (phospho Ser257) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-Caspase 6 (phospho Ser257) Antibody (A94575)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Anti-Caspase 6 (phospho Ser257) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 3: Immunofluorescence - Anti-Caspase 6 (phospho Ser257) Antibody (A94575)

Immunofluorescence analysis of HUVEC cells using Anti-Caspase 6 (phospho Ser257) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.