Principle of Assay
Caspase 2 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-VDQQD-pNA (acetyl-Val-Asp-Gln-Gln-Asp p-nitroanilide) by caspase 2, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm.