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Anti-CARS Antibody [ARC2504] (A305788)

Western Blot - Anti-CARS Antibody [ARC2504] (A305788) - Antibodies.com Immunohistochemistry - Anti-CARS Antibody [ARC2504] (A305788) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC2504] antibody to CARS for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-CARS Antibody [ARC2504]
Description
Rabbit monoclonal [ARC2504] antibody to CARS.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 650-748 of human CARS (P49589).
Sequence
EREEKRRVEEEKRKKKEEAARRKQEQEAAKLAKMKIPPSEMFLSETDKYSKFDENGLPTHDMEGKELSKGQAKKLKKLFEAQEKLYKEYLQMAQNGSFQ
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2504
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
85 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CysRS, Cysteine tRNA ligase, Cysteine--tRNA ligase, Cysteinyl tRNA synthetase, cytoplasmic, Cysteinyl-tRNA synthetase, cytoplasmic, MGC:11246, SYCC_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CARS Antibody [ARC2504] (A305788) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CARS Antibody [ARC2504] (A305788)
Western blot analysis of extracts of various cell lines, using Anti-CARS Antibody [ARC2504] (A305788) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry - Anti-CARS Antibody [ARC2504] (A305788) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-CARS Antibody [ARC2504] (A305788)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-CARS Antibody [ARC2504] (A305788) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Publishing research using Anti-CARS Antibody [ARC2504] (A305788)? Please let us know so that we can list the citation on this page.

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