Anti-CaMKII Antibody [22B1] (A305070)

Name: Anti-CaMKII Antibody [22B1]
Brand: Antibodies.com
SKU: A305070
Price: 465.0 USD
Availability: InStock

Enlarge Image

$465

Mouse monoclonal [22B1] antibody to CaMKII for WB, IHC, ICC/IF, IP and ELISA.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-CaMKII Antibody [22B1]

Description

Mouse monoclonal [22B1] antibody to CaMKII.

Specificity

This antibody detects phosphorylated CaMKII from rat tissues. This antibody is specific for a and beta subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in beta).

Applications

WB, IHC, ICC/IF, IP, ELISA

Dilutions

WB: 1:1,000, IHC: 1:100, ICC/IF: 1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide to Rat CaMKII.

Host

Mouse

Clonality

Monoclonal

Clone ID

22B1

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Calcium/calmodulin dependent protein kinase II alpha, Calcium/calmodulin dependent protein kinase II beta, Calcium/calmodulin dependent protein kinase II delta, Calcium/calmodulin dependent protein kinase II gamma, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II, CaM kinase II alpha, CaM kinase II beta, CaM kinase II delta, CaM kinase II gamma, CaM kinase II subunit alpha, CaMK-II subunit alpha, CAMK2, Camk2a, CAMK2B, CAMK2D, CAMK2G, CAMKA, KCC2A_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

Enlarge Image

Figure 1: Western Blot - Anti-CaMKII Antibody [22B1] (A305070)

Western blot analysis of mouse Ventricle lysates showing detection of CaMKII protein using Anti-CaMKII Antibody [22B1] (A305070) at 1:1,000. Analysis of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-CaMKII Antibody [22B1] (A305070)

Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-CaMKII Antibody [22B1] (A305070) at 1:5,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Magnification: 40x.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-CaMKII Antibody [22B1] (A305070)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-CaMKII Antibody [22B1] (A305070) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Muscle, hair follicle, epidermis. Backskin obtained from transgenic mice.

Enlarge Image

Figure 4: Immunofluorescence - Anti-CaMKII Antibody [22B1] (A305070)

Immunocytochemistry/Immunofluorescence analysis of rat dissociated hippocampal neurons, fixed in cold 4% paraformaldehyde / 0.2% glutaraldehyde in 0.1M sodium phosphate buffer, using Anti-CaMKII Antibody [22B1] (A305070), at 1:1,000 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at room temperature. Magnification: 10X.

Publishing research using Anti-CaMKII Antibody [22B1] (A305070)? Please let us know so that we can list the citation on this page.