Anti-Calponin 2 Antibody (A82867)

Name: Anti-Calponin 2 Antibody
Brand: Antibodies.com
SKU: A82867
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to Calponin 2 for ELISA, WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Calponin 2 Antibody

Description

Goat polyclonal antibody to Calponin 2.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:32,000, WB: 0.03-0.1µg/ml, IHC: 2.5µg/ml

Reactivity

Human

Cross Reactivity

This antibody is not expected to cross react with CCN3.

Immunogen

Synthetic peptide corresponding to the internal region (near C terminus) of human Calponin 2.

Sequence

C-DPGEVPEYPPYYQEE

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

35 kDa

Predicted MW

33.7kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Calponin H2, Calponin H2, smooth muscle, Calponin-2, CNN2, CNN2_HUMAN, Neutral calponin, smooth muscle

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Calponin 2 Antibody (A82867)

Calponin 2 expression in HepG2 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Calponin 2 Antibody (A82867) at 0.03µg/ml and detected by chemiluminescence.

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Figure 2: Immunohistochemistry - Anti-Calponin 2 Antibody (A82867)

Calponin 2 expression in Human Placenta analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Calponin 2 Antibody (A82867) at 2.5µg/ml and revealed with alkaline phosphatase (AP).

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