Anti-Calpain 2 Antibody (A14127)

Name: Anti-Calpain 2 Antibody
Brand: Antibodies.com
SKU: A14127
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Calpain 2 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Calpain 2 Antibody

Description

Rabbit polyclonal antibody to Calpain 2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 520-700 of human Calpain 2 (NP_001739.2).

Sequence

LEEFDISEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARFADDQLIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIELDLISWLCFSVL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Calcium activated neutral proteinase, Calcium activated neutral proteinase 2, Calcium-activated neutral protease 2, catalytic subunit, Calcium-activated neutral proteinase 2, CALP80, Calpain 2 (m/II) large subunit, Calpain 2 catalytic subunit, Calpain 2 large catalytic subunit, Calpain 2 large subunit, Calpain 2, large [catalytic] subunit, Calpain large polypeptide L2, Calpain M type, Calpain M-type, Calpain-2 catalytic subunit, Calpain-2 large subunit, Calpain2, CAN2_HUMAN, CANP 2, CANP L2, CANP2, CANPL 2, CANPL2, CANPml, Capa2, CAPN 2, CAPN2, FLJ39928, M calpain, M calpin, M type, M-calpain, mCANP, Millimolar calpain, Millimolar-calpain

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Calpain 2 Antibody (A14127)

Western blot analysis of extracts of various cell lines, using Anti-Calpain 2 Antibody (A14127). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.

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Figure 2: Western Blot - Anti-Calpain 2 Antibody (A14127)

Western blot analysis of extracts of various cell lines, using Anti-Calpain 2 Antibody (A14127) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 3: Immunohistochemistry - Anti-Calpain 2 Antibody (A14127)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Calpain 2 Antibody (A14127) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Calpain 2 Antibody (A14127)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Calpain 2 Antibody (A14127) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.