Synthetic peptide derived from human Calnexin (amino acids 543-592).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.
Molecular Weight
67kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CALX_HUMAN, CANX, CNX, FLJ26570, Histocompatibility complex class I antigen binding protein p88, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
Figure 1: Western Blot - Anti-Calnexin Antibody (A94879)
Western blot analysis of lysates from HeLa cells, treated with EGF 200ng/ml 30' using Anti-Calnexin Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Anti-Calnexin Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunofluorescence analysis of HeLa cells using Anti-Calnexin Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Figure 4: Western Blot - Anti-Calnexin Antibody (A94879)
Western blot analysis of various cells using Anti-Calnexin Antibody at 1:1,000 (4°C overnight). Goat Anti-Rabbit IgG (IRDye 800) was used as a secondary at 1:5,000 (25°C, 1 hour).
Immunohistochemical analysis of paraffin-embedded human uterus cancer tissue using Anti-Calnexin Antibody at 1:200 (4°C overnight). Negative control was secondary antibody only.
Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using Anti-Calnexin Antibody at 1:200 (4°C overnight). Negative control was secondary antibody only.
Immunofluorescence analysis of rat spleen tissue using Anti-Calnexin Antibody (red) at 1:200 (4°C overnight). Cy3 labelled secondary antibody was used at 1:300 (RT 50min). Panel A: Target. Panel B: DAPI. Panel C: Merge.
Immunofluorescence analysis of rat lung tissue using Anti-Calnexin Antibody (red) at 1:200 (4°C overnight). Cy3 labelled secondary antibody was used at 1:300 (RT 50min). Panel A: Target. Panel B: DAPI. Panel C: Merge.
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