Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CALX_HUMAN, CANX, CNX, FLJ26570, Histocompatibility complex class I antigen binding protein p88, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
Figure 1: Western Blot - Anti-Calnexin Antibody (A87725)
Western blot analysis of extracts of various cell lines, using Anti-Calnexin Antibody (A87725) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 2: Western Blot - Anti-Calnexin Antibody (A87725)
Western blot analysis of extracts of various cell lines, using Anti-Calnexin Antibody (A87725) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of PC-12 cells using Anti-Calnexin Antibody (A87725) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 4: Western Blot - Anti-Calnexin Antibody (A87725)
Immunoprecipitation analysis of 300µg extracts of NIH/3T3 cells using 3µg of Anti-Calnexin Antibody (A87725). This Western blot was performed on the immunoprecipitate using Anti-Calnexin Antibody (A87725) at a dilution of 1:500.
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