Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein A purification.
Concentration
1 mg/ml
Molecular Weight
~90 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CALX_HUMAN, CANX, CNX, FLJ26570, Histocompatibility complex class I antigen binding protein p88, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Calnexin Antibody (A305107), at 1:80 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum membrane. Melanosome. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Calnexin-CT Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol at -20°C for 10 minutes, using Anti-Calnexin Antibody (A305107), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at room temperature in dark. Localization: Nuclear staining, cytoplasmic staining.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution. The Primary Antibody used was Anti-Calnexin Antibody (A305107) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at room temperature. Localization: Hair Follicles, Basal cells in epidermis, and second layer of epidermis.
Figure 4: Western Blot - Anti-Calnexin Antibody (A305107)
Western blot analysis of rat tissue mix showing detection of Calnexin-CT protein using Anti-Calnexin Antibody (A305107) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Calnexin Antibody (A305107), at 1:80 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum membrane. Melanosome. Magnification: 100x. Heat Shocked at 42°C for 1h.
![Immunohistochemistry - Anti-Calnexin Antibody [CANX/1541] (A250400) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250400_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Calnexin Antibody [CANX/1541] - BSA and Azide free (A253580) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253580_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Calnexin Antibody [CANX/1543] (A250401) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250401_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Calnexin Antibody [CANX/1543] - BSA and Azide free (A253581) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253581_1.jpg?profile=product_alternative)