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Anti-Calmodulin 1 / 2 / 3 Antibody (A88516)

Western Blot - Anti-Calmodulin 1 / 2 / 3 Antibody (A88516) - Antibodies.com Immunohistochemistry - Anti-Calmodulin 1 / 2 / 3 Antibody (A88516) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Calmodulin 1 / 2 / 3 for WB and IHC.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Calmodulin 1 / 2 / 3 Antibody
Description
Rabbit polyclonal antibody to Calmodulin 1 / 2 / 3.
Applications
WB, IHC
Dilutions
WB: 1:100-1:500, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 80-149 of human CALM3 (NP_005175.2).
Sequence
TDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
17 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Calmodulin 1 / 2 / 3 Antibody (A88516) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Calmodulin 1 / 2 / 3 Antibody (A88516)
Western blot analysis of extracts of various cell lines, using Anti-Calmodulin 1 / 2 / 3 Antibody (A88516) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry - Anti-Calmodulin 1 / 2 / 3 Antibody (A88516) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Calmodulin 1 / 2 / 3 Antibody (A88516)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Calmodulin 1 / 2 / 3 Antibody (A88516) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Publishing research using Anti-Calmodulin 1 / 2 / 3 Antibody (A88516)? Please let us know so that we can list the citation on this page.
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