Anti-Calmegin Antibody [ARC2214] (A305871)

Name: Anti-Calmegin Antibody [ARC2214]
Brand: Antibodies.com
SKU: A305871
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2214] antibody to Calmegin for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Calmegin Antibody [ARC2214]

Description

Rabbit monoclonal [ARC2214] antibody to Calmegin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 180-350 of human Calmegin (O14967).

Sequence

FGPDKCGEDYKLHFIFRHKHPKTGVFEEKHAKPPDVDLKKFFTDRKTHLYTLVMNPDDTFEVLVDQTVVNKGSLLEDVVPPIKPPKEIEDPNDKKPEEWDERAKIPDPSAVKPEDWDESEPAQIEDSSVVKPAGWLDDEPKFIPDPNAEKPDDWNEDTDGEWEAPQILNPA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2214

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Calmegin precursor, Calnexin-T, Clgn, CLGN_HUMAN, MEG 1 antigen, OTTHUMP00000164610

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Calmegin Antibody [ARC2214] (A305871)

Western blot analysis of extracts of various cell lines, using Anti-Calmegin Antibody [ARC2214] (A305871) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-Calmegin Antibody [ARC2214] (A305871)

Western blot analysis of extracts of PC-3 cells, using Anti-Calmegin Antibody [ARC2214] (A305871) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-Calmegin Antibody [ARC2214] (A305871)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-Calmegin Antibody [ARC2214] (A305871) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Calmegin Antibody [ARC2214] (A305871)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-Calmegin Antibody [ARC2214] (A305871) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.