Mouse monoclonal [CALD1/820] antibody to Caldesmon.
Specificity
This antibody recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (12080kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This antibody recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
Applications
WB, IHC-P
Dilutions
WB: 2-4 µg/ml, IHC-P: 0.25-0.5 µg/ml
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Rat.
Immunogen
Recombinant full-length human CALD1 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
CALD1/820
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
150 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-Caldesmon Antibody [CALD1/820] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.