Anti-CACNB2 Antibody [N8b/1] (A304748)

Name: Anti-CACNB2 Antibody [N8b/1]
Brand: Antibodies.com
SKU: A304748
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [N8b/1] antibody to CACNB2 for WB, IHC, ICC/IF, IP and Antibody Microarray.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-CACNB2 Antibody [N8b/1]

Description

Mouse monoclonal [N8b/1] antibody to CACNB2.

Applications

WB, IHC, ICC/IF, IP, Antibody Microarray

Dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with Cav beta 1, Cav beta 3, or Cav beta 4.

Immunogen

Synthetic peptide amino acids 189-205 of rat CavBeta2.

Host

Mouse

Clonality

Monoclonal

Clone ID

N8b/1

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~78 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This antibody was formely sold under the Clone ID S8B-1.

Synonyms

AW060387, Ca(V) beta 2, CAB2, CACB2_HUMAN, CACNLB2, Calcium channel voltage dependent subunit beta 2, Calcium channel voltage-dependent subunit beta 2, Calcium channel, voltage dependent, beta 2 subunit, CAVB2, Cavbeta2, Cchb2, FLJ23743, Lambert Eaton Myasthenic syndrome antigen, Lambert Eaton myasthenic syndrome antigen B, Lambert-Eaton myasthenic syndrome antigen B, MGC129334, MGC129335, Myasthenic (Lambert Eaton) syndrome antigen B, Myasthenic syndrome antigen B, MYSB, Voltage dependent L type calcium channel subunit beta 2, Voltage-dependent L-type calcium channel subunit beta-2

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Immunofluorescence - Anti-CACNB2 Antibody [N8b/1] (A304748)

Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol for 10 minutes at -20°C, using Anti-CACNB2 Antibody [N8b/1] (A304748), at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: All cells positive. Bright dottiness located throughout cytoplasm and in nuclei.

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Figure 2: Western Blot - Anti-CACNB2 Antibody [N8b/1] (A304748)

Western blot analysis of human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Anti-CACNB2 Antibody [N8b/1] (A304748) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Figure 3: Immunohistochemistry - Anti-CACNB2 Antibody [N8b/1] (A304748)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-CACNB2 Antibody [N8b/1] (A304748) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: All nuclei. Some nuclei also staining higher up in epidermis.

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Figure 4: Immunofluorescence - Anti-CACNB2 Antibody [N8b/1] (A304748)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-CACNB2 Antibody [N8b/1] (A304748), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav beta 2 Antibody (D) Composite.

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