Anti-CACNA1H Antibody [N55/10] (A304750)

Name: Anti-CACNA1H Antibody [N55/10]
Brand: Antibodies.com
SKU: A304750
Price: 520.0 USD
Availability: InStock

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$520

Mouse monoclonal [N55/10] antibody to CACNA1H for WB, IHC, ICC/IF, IP and Antibody Microarray.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-CACNA1H Antibody [N55/10]

Description

Mouse monoclonal [N55/10] antibody to CACNA1H.

Applications

WB, IHC, ICC/IF, IP, Antibody Microarray

Dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with Cav1.3.

Immunogen

Fusion protein amino acids 1019-1293 (II-III loop) of human Cav3.2.

Host

Mouse

Clonality

Monoclonal

Clone ID

N55/10

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~260 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This antibody was formely sold under the Clone ID S55-10.

Synonyms

Low-voltage-activated calcium channel alpha1 3.2 subunit, Voltage-dependent T-type calcium channel subunit alpha-1H, Voltage-gated calcium channel subunit alpha Cav3.2

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-CACNA1H Antibody [N55/10] (A304750)

Western blot analysis of rat brain membrane lysate (native) showing detection of ~261 kDa Cav3.2 protein using Anti-CACNA1H Antibody [N55/10] (A304750) at 1:1,000 for 2 hours at room temperature. Block: 2% Skim Milk + 2% BSA in TBST. The secondary antibody used was Anti-Mouse: HRP at 1:4000. Predicted/Observed Size: ~261 kDa.

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Immunofluorescence - Anti-CACNA1H Antibody [N55/10] (A304750)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-CACNA1H Antibody [N55/10] (A304750), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav3.2 Antibody (D) Composite.

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Immunohistochemistry - Anti-CACNA1H Antibody [N55/10] (A304750)

Immunohistochemistry analysis of human frozen brain section, fixed in 10% formalin solution for 12-24 hours at room temperature. The Primary Antibody used was Anti-CACNA1H Antibody [N55/10] (A304750) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature.

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Immunohistochemistry - Anti-CACNA1H Antibody [N55/10] (A304750)

Immunohistochemistry analysis of human hippocampus, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-CACNA1H Antibody [N55/10] (A304750) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature.

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