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Anti-c-Myc (phospho Thr58) Antibody (A12454)

Western Blot - Anti-c-Myc (phospho Thr58) Antibody (A12454) - Antibodies.com Immunohistochemistry - Anti-c-Myc (phospho Thr58) Antibody (A12454) - Antibodies.com Immunohistochemistry - Anti-c-Myc (phospho Thr58) Antibody (A12454) - Antibodies.com Immunohistochemistry - Anti-c-Myc (phospho Thr58) Antibody (A12454) - Antibodies.com
$435
Product Datasheet

Rabbit polyclonal antibody to c-Myc (phospho Thr58) for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Email
orders@antibodies.com

Name
Anti-c-Myc (phospho Thr58) Antibody
Description
Rabbit polyclonal antibody to c-Myc (phospho Thr58).
Applications
WB, IHC
Dilutions
WB: 1:100-1:500, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around T58 of mouse MYC (NP_001170823.1).
Sequence
LPTPP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
62 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AU016757, Avian myelocytomatosis viral oncogene homolog, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39, MRTL, Myc, Myc Epitope Tag, Myc protein, Myc proto oncogene protein, Myc proto-oncogene protein, myc related translation/localization regulatory factor, myc-related translation/localization regulatory factor, Myc2, MYCC, MYC_HUMAN, Myelocytomatosis oncogene, Niard, Nird, oncogene c-Myc, Oncogene Myc, OTTHUMP00000158589, Proto-oncogene c-Myc, Protooncogene homologous to myelocytomatosis virus, RNCMYC, Transcription factor p64, Transcriptional regulator Myc-A, v myc avian myelocytomatosis viral oncogene homolog, v myc myelocytomatosis viral oncogene homolog, V-Myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-c-Myc (phospho Thr58) Antibody (A12454) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-c-Myc (phospho Thr58) Antibody (A12454)
Western blot analysis of extracts of 293T cells, using Anti-c-Myc (phospho Thr58) Antibody (A12454) at 1:500 dilution. 293T cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry - Anti-c-Myc (phospho Thr58) Antibody (A12454) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-c-Myc (phospho Thr58) Antibody (A12454)
Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-c-Myc (phospho Thr58) Antibody (A12454) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-c-Myc (phospho Thr58) Antibody (A12454) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-c-Myc (phospho Thr58) Antibody (A12454)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-c-Myc (phospho Thr58) Antibody (A12454) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-c-Myc (phospho Thr58) Antibody (A12454) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-c-Myc (phospho Thr58) Antibody (A12454)
Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-c-Myc (phospho Thr58) Antibody (A12454) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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