Anti-c-Jun (phospho Thr91) Antibody [ARC1548] (A308990)

Name: Anti-c-Jun (phospho Thr91) Antibody [ARC1548]
Brand: Antibodies.com
SKU: A308990
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC1548] antibody to c-Jun (phospho Thr91) for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-c-Jun (phospho Thr91) Antibody [ARC1548]

Description

Rabbit monoclonal [ARC1548] antibody to c-Jun (phospho Thr91).

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around T91 of human c-Jun (P05412).

Sequence

TTTPT

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1548

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

43 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Activator protein 1, AP 1, AP1, cJun, Enhancer Binding Protein AP1, JUN, Jun Activation Domain Binding Protein, Jun oncogene, JUN protein, Jun proto oncogene, JUNC, JUN_HUMAN, Oncogene JUN, p39, Proto oncogene c jun, Proto oncogene cJun, Proto-oncogene c-jun, Transcription Factor AP 1, Transcription factor AP-1, Transcription Factor AP1, V jun avian sarcoma virus 17 oncogene homolog, V jun sarcoma virus 17 oncogene homolog, V jun sarcoma virus 17 oncogene homolog (avian), V-jun avian sarcoma virus 17 oncogene homolog, vJun Avian Sarcoma Virus 17 Oncogene Homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-c-Jun (phospho Thr91) Antibody [ARC1548] (A308990)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-c-Jun (phospho Thr91) Antibody [ARC1548] (A308990) at 1:1,000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 ug/mL) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-c-Jun (phospho Thr91) Antibody [ARC1548] (A308990)

Western blot analysis of extracts of 293T cells, using Anti-c-Jun (phospho Thr91) Antibody [ARC1548] (A308990) at 1:1,000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 3: Immunohistochemistry - Anti-c-Jun (phospho Thr91) Antibody [ARC1548] (A308990)

Immunohistochemistry analysis of paraffin-embedded human breast tissue using Anti-c-Jun (phospho Thr91) Antibody [ARC1548] (A308990) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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