Anti-c-Fos Antibody (A12562)

Name: Anti-c-Fos Antibody
Brand: Antibodies.com
SKU: A12562
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to c-Fos for WB and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-c-Fos Antibody

Description

Rabbit polyclonal antibody to c-Fos.

Applications

WB, IP

Dilutions

WB: 1:500-1:1,000, IP: 1:500-1:1,000

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human c-Fos (NP_005243.1).

Sequence

MMFSGFNADYEASSSRCSSASPAGDSLSYYHSPADSFSSMGSPVNAQDFCTDLAVSSANFIPTVTAISTSPDLQWLVQPALVSSVAPSQTRAPHPFGVPAPSAGAYSRAGVVKT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 - 60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Activator protein 1, AP 1, C FOS, Cellular oncogene c fos, Cellular oncogene fos, FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS), FBJ murine osteosarcoma viral oncogene homolog, FBJ murine osteosarcoma viral v fos oncogene homolog, FBJ Osteosarcoma Virus, FOS, FOS protein, FOS_HUMAN, G0 G1 switch regulatory protein 7, G0/G1 switch regulatory protein 7, G0S7, Oncogene FOS, p55, proto oncogene c Fos, Proto oncogene protein c fos, Proto-oncogene c-Fos, v fos FBJ murine osteosarcoma viral oncogene homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-c-Fos Antibody (A12562)

Western blot analysis of extracts of HeLa cells, using Anti-c-Fos Antibody (A12562) at 1:1,000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-c-Fos Antibody (A12562)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-c-Fos Antibody (A12562). This Western blot was performed on the immunoprecipitate using Anti-c-Fos Antibody (A12562) at a dilution of 1:1000.

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