Anti-c-Fos Antibody [4A3] (A305157)

Name: Anti-c-Fos Antibody [4A3]
Brand: Antibodies.com
SKU: A305157
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [4A3] antibody to c-Fos for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-c-Fos Antibody [4A3]

Description

Mouse monoclonal [4A3] antibody to c-Fos.

Specificity

Observed molecular weight of band is higher than predicted 41 kDa due to post translational modifications.

Applications

WB, IHC

Dilutions

WB: 1:1,000, IHC: 1:100

Reactivity

Human, Rat

Immunogen

Synthetic peptide from the N-terminal of Human c-Fos (aa. 280-380).

Host

Mouse

Clonality

Monoclonal

Clone ID

4A3

Isotype

IgG2

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

62 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Activator protein 1, AP 1, C FOS, Cellular oncogene c fos, Cellular oncogene fos, FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS), FBJ murine osteosarcoma viral oncogene homolog, FBJ murine osteosarcoma viral v fos oncogene homolog, FBJ Osteosarcoma Virus, FOS, FOS protein, FOS_HUMAN, G0 G1 switch regulatory protein 7, G0/G1 switch regulatory protein 7, G0S7, Oncogene FOS, p55, proto oncogene c Fos, Proto oncogene protein c fos, Proto-oncogene c-Fos, v fos FBJ murine osteosarcoma viral oncogene homolog

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-c-Fos Antibody [4A3] (A305157)

Western blot analysis of human Serum starved HeLa cells treated with PMA showing detection of 41 kDa c-fos protein using Anti-c-Fos Antibody [4A3] (A305157) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: Serum starved HeLa cell lysates. Lane 3: Serum starved HeLa cells treated with PMA (Phorbol 12-myristate 13-acetate). Load: 10 µg. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 41 kDa. Other Band(s): 62 kDa. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.

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Figure 2: Immunohistochemistry - Anti-c-Fos Antibody [4A3] (A305157)

Immunohistochemistry analysis of rat kidney. The Primary Antibody used was Anti-c-Fos Antibody [4A3] (A305157) at 1:100 for Overnight at 4°C, then 30 minutes at 37°C. The secondary antibody used was Goat Anti-Mouse IgG (H+L): FITC for 45 minutes at 37°C. Counterstain: DAPI for 3 min at room temperature. Magnification: 20X.

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