Rabbit polyclonal antibody to C / EBP-α (Phospho-Ser21)
Specificity
The antibody detects endogenous levels of C / EBP-α only when phosphorylated at serine 21.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Serine 21 (L-Q-S(p)-P-P) derived from Human C / EBP-α.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
Capsid protein, core, Core and e antigen, Core antigen, Core protein, HBc, HBcAg, HBVgp4, Hepatitis B core antigen, Hepatitis B Virus Core Antigen, p21.5, precore/core protein
Western blot analysis of extracts from HepG2 cells treated with EGF using C/EBP-α (phospho-Ser21) Antibody #11648.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using C/EBP-α (phospho-Ser21) antibody #11648 (left)or the same antibody preincubated with blocking peptide (right).
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