Anti-Bub1 Antibody [ARC59688] (A309294)

Name: Anti-Bub1 Antibody [ARC59688]
Brand: Antibodies.com
SKU: A309294
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC59688] antibody to Bub1 for WB.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Bub1 Antibody [ARC59688]

Description

Rabbit monoclonal [ARC59688] antibody to Bub1.

Applications

Dilutions

WB: 1:500-1:1,000

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human BUB1.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC59688

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

122 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BUB1 budding uninhibited by benzimidazoles 1 homolog, BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast), BUB1 mitotic checkpoint serine/threonine kinase, BUB1, S. cerevisiae, homolog of, BUB1A, BUB1L, BUB1_HUMAN, Budding uninhibited by benzimidazoles 1 (yeast homolog), Budding uninhibited by benzimidazoles 1 homolog, Budding uninhibited by benzimidazoles 1, S. cerevisiae, homolog of, hBUB1, Homolog of mitotic checkpoint gene BUB1, Mitotic checkpoint gene BUB1, Mitotic checkpoint serine/threonine protein kinase BUB1, Mitotic checkpoint serine/threonine-protein kinase BUB1, Mitotic spindle checkpoint kinase, Putative serine/threonine protein kinase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Bub1 Antibody [ARC59688] (A309294)

Western blot analysis of HEL, using Anti-Bub1 Antibody [ARC59688] (A309294) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-Bub1 Antibody [ARC59688] (A309294)

Western blot analysis of extracts from wild type(WT) and BUB1 Rabbit mAb knockdown (KD) 293T(KD) cells, using Anti-Bub1 Antibody [ARC59688] (A309294) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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