Recombinant human monoclonal [ICT-01] antibody to BTN1A1.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human
Host
Human
Clonality
Monoclonal
Clone ID
ICT-01
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 146.2 kDa.
Purity
> 95% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
BT, BT1A1_HUMAN, BTN, BTN1, butyrophilin, Butyrophilin subfamily 1 member A1, butyrophilin, subfamily 1, member A1
SDS-PAGE analysis of Anti-BTN1A1 Antibody [ICT-01] - Low endotoxin, Azide free (A323905) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
Human BTN3 HEK293 cells were stained with Anti-BTN1A1 Antibody [ICT-01] - Low endotoxin, Azide free (A323905) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 8.4730 nM.
Anti-BTN1A1 Antibody [ICT-01] - Low endotoxin, Azide free (A323905) captured on CM5 chip via Anti-Human IgG Fc Antibodies surface, can bind human BTN3A1 / BTF5 / CD277, hFc Tag with an affinity constant of 6.96E-10M as determined in a SPR assay.
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