Anti-BTAF1 Antibody (A14990)

Name: Anti-BTAF1 Antibody
Brand: Antibodies.com
SKU: A14990
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to BTAF1 for WB, IHC, IP and ChIP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-BTAF1 Antibody

Description

Rabbit polyclonal antibody to BTAF1.

Applications

WB, IHC, IP, ChIP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:20-1:50, ChIP: 1:20-1:100

Reactivity

Human, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1600-1849 of human BTAF1 (NP_003963.1).

Sequence

LAVQNSSLHDIQHAPKLSALKQLLLDCGLGNGSTSESGTESVVAQHRILIFCQLKSMLDIVEHDLLKPHLPSVTYLRLDGSIPPGQRHSIVSRFNNDPSIDVLLLTTHVGGLGLNLTGADTVVFVEHDWNPMRDLQAMDRAHRIGQKRVVNVYRLITRGTLEEKIMGLQKFKMNIANTVISQENSSLQSMGTDQLLDLFTLDKDGKAEKADTSTSGKASMKSILENLSDLWDQEQYDSEYSLENFMHSLK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

250 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

ATP-dependent helicase BTAF1, B-TFIID transcription factor-associated 170 kDa subunit, TAF(II)170, TAF-172, TAF172, TATA-binding protein-associated factor 172, TBP-associated factor 172

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-BTAF1 Antibody (A14990)

Western blot analysis of extracts of various cell lines, using Anti-BTAF1 Antibody (A14990) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit.

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Western Blot - Anti-BTAF1 Antibody (A14990)

Immunoprecipitation analysis of 150µg extracts of HeLa cells using 3µg of Anti-BTAF1 Antibody (A14990). This Western blot was performed on the immunoprecipitate using Anti-BTAF1 Antibody (A14990) at a dilution of 1:500.

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Chromatin Immunoprecipitation - Anti-BTAF1 Antibody (A14990)

Chromatin immunoprecipitation (ChIP) analysis of extracts of HeLa cells, using Anti-BTAF1 Antibody (A14990) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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