Anti-BRSK1 Antibody (A304939)

Name: Anti-BRSK1 Antibody
Brand: Antibodies.com
SKU: A304939
Price: 500.0 USD
Availability: InStock

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$500

Rabbit polyclonal antibody to BRSK1 for WB, ICC/IF and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-BRSK1 Antibody

Description

Rabbit polyclonal antibody to BRSK1.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:1,000, ICC/IF: 1:100, IHC: 1:50

Reactivity

Human, Mouse

Immunogen

Synthetic peptide from the mid-protein of Human BRSK1.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BR serine/threonine kinase 1, BR serine/threonine protein kinase 1, BR serine/threonine-protein kinase 1, Brain selective kinase 1, Brain-specific serine/threonine-protein kinase 1, BRSK 1, BRSK1_HUMAN, FLJ43009, hSAD1, KIAA1811, Protein kinase SAD1A, SAD 1, SAD1, SAD1 homolog, SAD1 kinase, SAD1A, SADB, SadB kinase short isoform, Serine/threonine kinase SAD B, Serine/threonine-protein kinase SAD-B, Synapses of Amphids Defective homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunohistochemistry - Anti-BRSK1 Antibody (A304939)

Immunohistochemistry analysis of human brain, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-BRSK1 Antibody (A304939) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X.

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Figure 2: Western Blot - Anti-BRSK1 Antibody (A304939)

Western blot analysis of mouse brain cell lysates showing detection of ~85.1 kDa BRSK1 protein using Anti-BRSK1 Antibody (A304939) at 1:1,000 for 60 minutes at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~85.1 kDa.

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Figure 3: Immunofluorescence - Anti-BRSK1 Antibody (A304939)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-BRSK1 Antibody (A304939), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm, Nucleus. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BRSK1 Antibody (D) Composite.

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